河豚毒素的毒性效应研究

AbstractIn this study,healthy male mice without specific pathogen grade (SPF)ICR strainwere selected as subjects.The detoxification effect of recombinant protein on TTXintoxication in mice was studied by gavage and intraperitoneal injection of TTX-recombinant protein mixture.The results showed that the mortality rate of mice was 60%within 2h after gavage of 350 g/kg BW,which was used as the TTX concentration of thecontrol group.All mice survived after gavage of the mixture of toxin and protein afterincubation,indicating that TTX was non-toxic after in vitro incubation of TTX andprotein.The survival of mice with intraperitoneal injection of TTX concentration of 7.5g/kg BW was prolonged,and the survival rate of mice with intraperitoneal injection ofthe mixture of toxin and protein after incubation was significantly increased,indicatingthat TTX significantly decreased after in vitro incubation of TTX and protein.Key words:TTX;Puffer fish;mice;TTX binding protein