茶树胶孢炭疽菌VPS29基因的功能分析

AbstractAnthracnose of tea plant is mainly caused by Colletotrichum gloeosporioides,which is the main disease of tea plant.The physiological metabolism of tea isseriously affected by the anthracnose,and this disease reduces the output and qualityof tea year by year.Therefore,to prevent and reduce tea anthracnose,differentmethods were needed according to the characteristics of pathogen.Vps29 is one of thesubunits of Retromer protein complex.It may involve in the process of C.gloeosporioides infecting tea trees and have important functions.To this end,In thisstudy,we deleted the /PS29 gene according to the homologous recombinationprinciple in C.gloeosporioides.The VPS29 knockout homologous recombinantfragment was amplified by double-joint PCR and transform to protoplast of C.gloeosporioides N425.Screening of the transformants with geneticin resistance andconfirming by PCR.A VPS29 null mutant named Avps29-7 was generated.Analysisof vegetative growth and sporulation of the Avps29-7 and we found that the growth ofthis mutant on solid medium was slower than that of wild-type.It is suggested thatVPS29 controlled the vegetative growth of C.gloeosporioides.Next step,furtherphenotypes will be analyzed to identify the entire biological function of /PS29 in C.gloeosporioides.Key words:tea plant;Colletotrichum gloeosporioides:Retromer complex;geneknockout