大黄鱼TRIM25基因在饵料中添加中草药后的表达变化

AbstractTheThe three fold motif protein 25(Tripartite motif-containing protein25,TRIM25)plays an important role in innate immune response.In thisexperiment,by adding different proportions of Chinese herbal compound(Radix pseudosciae,Radix Morindae,angelica,wolfberry,Poria,Atractylodes macrocephala)in large yellow croaker feed,30 days later,the kidney tissue of Pseudosciaena crocea was used as exper imentalmaterial.The relative expression of TRIM25 gene after feeding Chineseherbal medicine was analyzed by real-time quantitative PCR.Resultsshowed that compared with the control group,the expression of the genedecreased in all the exper imental groups (exceptgroup E2),the expressionlevel of E3 group decreased by 81%,followed by El group(68%),and theexpression level of E5 group was 654%higher than that of control group.Butthere was no significant difference among all groups (P greater than).ZeroPointZero FiveThis study provides basic data for the application ofChinese herbal medicine as feed additive in large yellow croaker feed,and provides a prel iminary research basis for Chinese herbal medicine asa fish immune enhancer.Keywords:Pseudosciaena crocea:Pseudosciaena heterophylla extract;TRIM25;