转植酸酶和抗粘液病毒基因猪

AbstractWith the development and widespread application of biological technology,transgenic animalstechnology continues to mature and widely used in medicine,production,food,etc.It brings hugeeconomic benefits to the society.Chinese has also won the breakthrough progress and gain a lot ofscientific fruits.Transgenic rabbit,chickens,pigs,cows and other animals have come out and they havefast growth,strong resistance and good fleshy.However,transgenic animals likely have a negativeimpact on environment and biodiversity.The security question of transgenic animals has alreadybecome the focus of the society and the biotech security has become increasingly fierce,and thecontroversy is mainly concentrated in ecological environment,food safety,etc.In order to verify whether transgenic pig have influence to the environment,especially theexogenous gene whether drift to other organisms'genome.The paper carrys on safety assessmenttechnology research of exogenous gene and transgenic pigs,focus on the key problems of safetyassessment research.Aimed at the impact of transgenic pigs to environmental,we detect the gene driftbetween transgenic pigs and no-transgenic pigs,transgenic pigs and surrounding environment.We try toexplore whether exogenous gene of transgenic pigs would be transferred to other organisms genome.1)Analysis on PCR detection ability of exogenous geneDiluted the known concentration plasmid which have been integrated exogenous gene and thenamplified exogenous gene by PCR.The resuLts showed that the detection ability of appA.CMV-appA.MX1、CMV-MX1 in PCR system were:2.4x10 copies/μuL、2.4x10'copies/L、2.4x102 copies/μL、2.4x103 copies/μL。2)Detection of gene drift between transgenic pigs and non-transgenic pigsExtracting DNA from placenta and blood of recipient pig and testing exogenous gene by PCR.TheresuLts showed that there is no exogenous gene.Choose four transgenic sows and four Large Whitepigs in its 2 months and kept in a corral.Then collected the blood of four Large White pigs after 3.5 7months and tested exogenous gene by PCR.The resuLts showed that there is no exogenous gene.3)Detection of exogenous gene in pig farm and soil around the pig farmExtracting DNA from feces in pig farm and confirming the inhibitory reaction of feces DNA toPCR.We amplified exogenous gene in 50uL amplification system with a certain amount of feces DNAto replace ddH2O.When we add0、luL、2.5μL、5μL、10μL、15uL,PCR detection ability were2.49×10copies/mL、2.49x10 copies/μL、2.49×10'copies/mL、2.49x103 copiesμL、0.We Extracted DNA fromfeces of transgenic pigsoil in pig farm and soil around the pig farm and tested exogenous gene by PCR.The resuLts showed that there is no exogenous gene.Key word:Transgenic pig,:appA:MY/:Environment Safety assessment:PCR