液质联用分析多肽

AbstractHigh-performance liquid chromatography-mass spectrometry (HPLC-MS)inconjunction with technology in recent years developed a new separation analysistechniques.It a very wide range of applications of separation methods -liquidchromatography with sensitive,proprietary,can provide information on molecularweight and structure of the combination of mass spectrometry has become animportant separation of modern analysis techniques.HPLC-MS provide lots ofvaluable data for production and scientific research,and solve manypreviously difficult problems.This papermainly explores the LC-MS conditions,andthe use of LC-ESI-MS instrument and analysis of impurities on the properties of theproduct to determine the nature of the polypeptide,hope to establish a complete setof liquid chromatography-mass spectrometry system.Key Words HPLC-MS,in combination with the condition,nature of theproduct identification,impurity analysis目录摘要aceme-em--m-e-m-e-m-----m-m.Abstract第一章前言….Π第二章实验部分.62.1实验目的…2.2实验原理…..62.3实验仪器与试剂.…82.4实验步骤.82.4.1管道连接.…..82.4.2流动性制备8