蒲地蓝消炎片中板蓝根有效成分(R，S)-告依春含量测定方法的研究

蒲地蓝消炎片中板蓝根有效成分(RS)-告依春含量测定方法的研究摘要目的创建蒲地蓝消炎片中(R,S)-告依春成份获取及含量测量的方法。方法本毕业论文所采用的获取方法是超声法。获取水溶液挑选工业甲醇，容积为25ml,时长30mi。所采用的测量方法是高效液相色谱法。以(R,S)-告依春为标准品，以工业甲醇-0.02%硫酸铵水溶液(7：93)为流动相，流动速度为0.8ml/min在242m波优点测量RS-告依春的含量。结论根据高效液相色谱法测出蒲地蓝消炎片中（®，S告依春的含量。结果本方法迅速简单，可重复性好，适用性强，适合于蒲地蓝消炎片R,S)-告依春含量的检测方法。关键词：蒲地蓝消炎片：(R,S-告依春：高效液相色谱法：含量测定AbstractObjective To establish a method for the extraction and content measurement of(R,S)-Jiaoyichun in Pudilan Xiaoyan tablets.Methods The acquisition method ofthis paper is ultrasonic method.The aqueous solution selected industrial methanolwas obtained,with a volume of 25ml and a duration of 30min.The measurement methodused is high performance liquid chromatography.(R,S)-Chuichun was used as thestandard substance,and industrial methanol-0.02%ammonium sulfate solution (7:93)was used as the mobile phase with a flow rate of 0.8ml/min.The content of RS-Chuichunwas measured at 242nm wave.Conclusion The content of (R,S)-Guiyichun in PudilanXiaoyan tablets was determined by HPLC.Results The method was rapid,simple,reproducible and suitable for the determination of (R,S)-Jiaoyichun in PudilanXiaoyan tablets.Key words:Pudilan xiaoyan Tablets:(R,S)-epigoitrin:HPLC:Determination of content0目口录、前言…1.1蒲地蓝消炎片及板蓝根概况1.2(R,S)-告依春的研究1.3本论文研究的意义2二、材料与仪器…2.1试剂与试药32.2仪器…三、方法.…43.1(R,S)-告依春含量测定方法.43.1.1色谱条件3.1.2对照品溶液制备3.1.3供试品溶液制备.。43.1.4对照药材制备43.2方法学考察3.2.1线性关系考察.43.2.2检测限和定量限53.2.3精密度试验53.2.4稳定性试验..63.2.5阴性对照试验.63.2.6重复性试验.73.2.7加样回收率试验.73.2.8样品含量的测定.83.3单因素考察方法….83.3.1R,S-告依春的提取83.3.2检测波长选择….93.3.3提取温度的考察.103.3.4柱温103.3.5流速的选择…103.4结果.103.4.1蒲地蓝消炎片中(R,S)-告依春的提取测定方法.10四、讨论114.】提取条件分析1们4.1.1蒲地蓝消炎片不同提取方法的分析.114.1.2提取温度的影响114.1.3提取时间的影响.1门4.2测定条件分析…124.2.】检测波长选择…124.2.2柱温的选择124.2.3流速的选择.12五、结论120六、板蓝根的研究进展…13七、板蓝根化学成分的研究13八、板蓝根化学成分提取方法的研究14九、板蓝根药理作用的研究…149.1抗内毒素作用149.2抗肿瘤作用.159.3抗病毒作用169.4板蓝根其它方面的作用…16十、板蓝根的研究展望…17致谢…18参考文献。19IV