重组人表皮生长因子原液车间工艺设计

Process design of recombinant human epidermal growth factorstock solution workshopAbstractHuman epidermal growth factor (hEGF)is a small molecular peptide composedof 53 amino acids,with a molecular weight of about 6000 Daltons and an isoelectricpoint of about 4.6.There are three pairs of disulfide bonds in the molecule,so it isphysical,chemical,and acidic The factors are relatively stable.hEGF has a widerange of biological effects and can strongly promote the growth of various epidermaltissues.It has been used in medicine to treat burns,ulcers,various types of trauma,and corneal injuries.EGF can also promote the metabolism of normal epidermal cells.Adding it to beauty skin care products can achieve whitening,anti-wrinkle,andanti-aging effects.Before the 1980s,the source of hEGF was mainly through tissueand body fluid extraction.Due to the extremely small content of hEGF,the extractioncost was high and the purity of the product was low,which limited the application ofhEGF.Since the 1980s,with the development of genetic engineering technology,people have achieved success in the production of hEGF through geneticrecombination technology,using E.coli,yeast,etc.,which has laid the foundation forthe industrial production of genetically recombinant human epidermal growth factor.Therefore,the production process in this design uses E.coli as the host strain,applieshigh-density fermentation technology,and produces rhEGF stock solution on a largescale through protein purification and other technologies.This design combines the actual situation of recombinant protein drugs in China.After consulting a large number of documents and standards,the process flowdrawing and workshop design have been completed,the material balance and the heatbalance have been calculated,and the design and selection of the main equipment.Keywords:Human epidermal growth factor;High density fermentation process:Workshop design