辣木黄酮和多糖提取方法及其含量影响因素的初步研究

The preliminary study on the influence factors of extraction methods andcontent of Flavones and Polysaccharide in Moringa OleiferaAbstractAim to stabilize and improve the products by effective content of Moringa oleifera,which is from the ecological park Yingdong of Shaoguan University in Guangdongplanting Moringa oleifera of two years.We found that the ethanol circumfluence forflavones determining and Phenol-sulferic acid method for Polysaccharide were the optimalextraction technology.Addition to this,the relationship of the Leaves,organ,producingarea,Harvest time,and levels under the management,position with the effectivecomponent content would be discussed.The results showed that:The optimal extraction technology of total flavones from the leaves of Moringaoleifera was:70%ethanol,20 times volume,80C,90 minutes and extract three times.Theyield of total flavones was 6.59%under this optimal extraction condition.The optimal extraction technology of polysaccharides from the leaves of Moringaoleifera was:15 times volume waters,90'C,120 minutes and extract three times.The yieldof polysaccharide content was 24.76%under this optimal extraction condition.The total flavones and polysaccharide content of Moringa oleifera were the highest onthe stronger leaves,which 45 days older.The total flavone was 6.11%and polysaccharidewas 21.97 on these leaves.Otherwise,these contents were less on the younger leavesand older leaves.Total flavones content of Moringa oleifera in different organs were showed that:theflower-petiole is most,root is least,and the variety bound were 0.53%~4.47%.And thepolysaccharide content on the roots were most,the petiole were least,and the varietybound were 8.16%~33.61%.The effective components on the different Producing area were:the total flavoneswere the highest in the materials from the west suburb-racing factory.In which the leaveswere 6.78%,petioles were 3.43%and stems were 2.66%.But the polysaccharide in theXinfeng factory was the highest levels.The leaves were 27.14%,petioles were 16.19 %and stems were 12.28%.The effective components were the least which from theYingdong ecological park of Shaoguan University.There was a difference of the effective components within different harvesting periodin our experiment.It's showed that the effective component content was the highestharvested in November.The total flavone of leaves were5.69%,the petiole were 3.13 %and stems were2.01 %The polysaccharides of leaves were 28.85%,the petioles were 9.712