IL-17及其受体通过Jagged-Notch信号通路调控

安微医科大学硕士学位论文结果基于内镜观察和声导抗检查确认成功建立免疫相关的SOM大鼠模型。1.SOM大鼠模型组的中耳积液L-17的含量显著高于正常组大鼠中耳灌洗液含量(P<0.05),而外周血浆中IL-17含量高于正常对照组外周血含量(P<0.05)2.基于光镜条件下的中耳组织的H©染色切片显示模型组鼓膜及血管周围有明显炎症细胞浸润，细胞的排列呈现出紊乱的特点，上皮细胞则出现坏死、脱落等非良性现象，粘液则为水肿状态，血管壁也呈现出增厚的特点。而正常对照组的H染色切片则并没有出现明显的炎症细胞浸润，上皮细胞则呈现出排列整齐的特点。3.Real Time PCR结果显示与对照组相比，PBS组、SOM组、SOM+IL-17组中IL-17、Notchl、Jagged1、DLLA的mRNA表达均升高(P<0.05),PBS组低于S0M+IL-17组，高于正常对照组(P<0.05)。4.Western B1ot结果显示与正常组相比，SOM组IL-l7、Notchl、Jaggedl、DLL4蛋白表达明显升高，PBS组和S0M+IL-17组各蛋白表达低于模型组(P<0.05)。结论S0M的免疫相关发病机制可能与IL-17密切相关，而IL-17是如何调节SOM的发展尚不明确：IL-17作为重要的免疫介质参与到S0M进展中，并且可能通过Notch信号通路调控Thl/Th2细胞失衡来实现。关键词分泌性中耳炎白介素-17 Notch信号通路Thl/Th2失衡IL-17 and its receptor regulate Th1/Th2 cell imbalance throughJagged-Notch signaling pathway to affect the pathogenesis ofsecretory otitis media in ratsAbstractBackground Secretory Otitis Media(SOM)is a non-suppurative inflammatory diseaseof the middle ear with the main characteristics of middle ear effusion and hearingloss.When hearing loss persists or recurs repeatedly,it seriously affects children'slanguage development,behavior development and personality formation,and finallyseriously affects patients'quality of life.At present,the specific mechanism ofautoimmune factors promoting SOM development is not clear.Th17 cells play animportant role in immune regulation,and IL-17 plays an important role in secretoryotitis media and tumor immunity through receptor specific binding.Normally,Th1/Th2 cells in the body are in a relatively balanced state.Once the balance betweenTh1/Th2 is broken,the dynamic balance of inflammatory factor network is likely to bedestroyed,thus causing the generation and development of many diseases.Based onthe research progress at home and abroad and the previous research basis of theresearch group,the following research hypothesis was proposed:IL-17 binding with2