芦荟通畅胶囊遗传毒性研究

芦荟通畅胶囊遗传毒性研究摘要目地：点评芦荟畅胶囊的基因遗传毒性：方式小白鼠淋巴肿瘤TK基因变异试验(mouselymphomaassaytkgenemutationtest)、伤寒沙门氏菌/修复基因突变试验(salmonellatyphimuriun/reversemutationasay)、Ames Assay)、脊髓芦荟流畅胶囊萃取液剂量组20、10、5、2.5、1.25mg/皿，在加上和不含有S9的条件下根据Ames试验和TK试验开展身体之外基因遗传毒性点评：设置芦荟开通胶囊混悬剂剂量组0.25、0.125、0.0625g/ml,根据骨髓细胞微核试验开展基因遗传毒性点评。在Ames试验中，设定记数TA97a、TA98、TA100、TA102四个标准试验。菌种在4个浓度值下，37、48h塑造后修复基因突变菌落总数：在MLA试验中，尺寸村庄的孔数、平板电脑高效率PE%、基因变异工作频率MF、及其小村落比例SC%:脊髓微核试验每一只小动物记数1000个嗜多染血细胞，其微核发病率为含微核的PCE千分率。结论：芦荟畅胶囊提取液Ams试验试验者各剂量组回变菌落总数都超过有机溶剂对比回变菌落总数的2倍，没有出现剂量-反映关联：MLA试验中，芦荟畅胶囊提取液各剂量组MF值与有机溶剂对照实验组较为差别无显著性差异，没有出现剂量-反映关联；骨髓细胞微核试验试验者各剂量组微核发病率无显著性差异P0.05),但雌和雄小白鼠阳性对照组均远远高于阴性对照组(P0.01)。结果芦荟流畅胶囊提取液Ames试验、MLA试验、骨髓细胞微核试验都未验出基因遗传毒性关键词：芦荟通畅胶囊；小鼠淋巴瘤TK基因突变实验：鼠伤寒沙门氏菌/回复突变试验：骨髓细胞微核试验AbstractObjective:To review the genotoxicity of Aloe Chang Capsule;White mouselymphoma TK gene mutation test (mouselymphomaassaytkgenemutationtest),salmonellatyphi/repairgenemutationtest(salmonellatyphimuriun/reversemutationasay),Ames Assay)and myelaloe Fluicapsules extract dose groups of 20,10,5,2.5,1.25mg/plate were assessed onextra-body genotoxicity according to Ames test and TK test with and without S9.Thedosage groups of aloe capsule suspension were 0.25,0.125 and 0.0625g/ml,and thegenogenotoxicity was evaluated according to micronucleus test of bone marrow cells.In Ames test,four standard tests,TA97a,TA98,TA100 and TA102,were set.The totalnumber of repaired mutant colonies after 37 and 48 h were constructed under 4concentration values.In the MLA experiment,the number of holes in the size village,the tablet high efficiency PE%,the working frequency of gene variation MF,and theproportion of small village SC%;Spinal micronucleus test counted 1000polychromatic blood cells per small animal,and the micronucleus incidence rate wasmicronucleus containing PCE thousand rate.Conclusions:The total number ofreverted colonies in Ames test of Aloe Chang capsule extract was more than 2 timesof the total number of reverted colonies in organic solvent,and there was nodose-response correlation.In MLA test,there was no significant difference in MFvalue between Aloe Chang capsule extract groups and organic solvent control group,and there was no dose-response correlation.There was no significant difference inmicronucleus incidence among different dose groups in bone marrow cellmicronucleus test (P0.05),but female and male mice in positive control group weremuch higher than that in negative control group (P0.01).Results No genotoxicity wasdetected in Ames test,MLA test and micronucleus test of bone marrow cells.Key words:Aloe smooth capsule;Mouse lymphoma TK gene mutation experiment;Salmonellatyphimurium/back mutation test;Bone marrow cell micronucleus test2目录一.前言…1.芦荟毒性作用研究...21.芦荟毒性效应2.芦荟中蒽醌的毒性作用机制.32.芦荟的临床不良反应41.皮肤不良反应2.胃肠道不良反应53.肝脏不良反应.54.肾脏不良反应65.心血管不良反应6二.材料与仪器61.样品.…62.主要仪器与试剂3.实验动物.三.实验方法....a .....1.样品制备1.MLA实验样品制备72.Ames实验样品制备3.骨髓微核试验样品制备2.剂量选择与受试物给予方式1.MLA实验剂量选择0.72.Ames实验剂量选择.83.骨髓微核试验剂量选择与受试物给予方式.83.小鼠淋巴瘤TK基因突变实验(MLA试验).81.培养液和培养条件.82.细胞复苏