中草药对大黄鱼谷胱甘肽还原酶基因表达的影响

AbstractGlutathione reductase is an antioxidant enzyme that exists primarily in theorganism and is involved in a variety of biological processes in the organism.In thisstudy,Larimichthys crocea was taken as the research subject,and the effect ofChinese herbal medicine on GR gene expression in L.crocea was studied by addingdifferent doses of Chinese herbal medicine into the basal feed.The expression level ofGR gene in spleen of large yellow croaker was detected by fluorescence real-timequantitative PCR at different doses.The results were as follows:compared with thecontrol group,the expression of GR gene in each experimental group decreased todifferent degrees,but there was no significant difference between each group (P0.05).The results of this study provided basic data for the application of Chineseherbal medicine as a bait additive in the feed of Chinese croaker,and also providedthe basis for the preliminary study of Chinese herbal medicine as an immune boosterfor fish.Key words:Chinese herbal medicine;Larimichthys crocea;glutathione reductase;gene expression