枣乌头酸酶的活性测定

枣乌头酸酶的活性测定摘要本次毕业设计，以枣和酸枣中的细胞浆的乌头酸酶的测定为出发点，分别对枣和酸枣的乌头酸酶的活性进行了测量和分析，并进行了比较，认为乌头酸酶的活性与枣和酸枣的质量有关。顺乌头酸酶属于铁硫蛋白，它的身体里有一个F.S.),可以将柠檬酸转化，经过三羧酸循环，最终形成异柠檬酸钠。AC0将柠檬酸转化为异柠檬酸，异柠檬酸上的氧化脱羧作用使得NADH的吸收峰在240m处得到了明显的增强。通过对上述结果的分析，得出了本文的主要结论。本课题组前期研究表明，在我国红枣生产上存在着一类具有一定代表性的“过渡类型”，这种“过渡类型”的“过渡类型”在红枣向柠檬酸的转变过程中发挥着关键作用。在此基础上，通过比较分析，阐明山楂与山楂的共性与差异，为山楂的选育与驯化奠定理论基础。关键词：枣：顺乌头酸酶：柠檬酸：异柠檬酸盐：三羧酸循环AbstractIn this graduation project,the measurement and analysis of the activity ofaconitidase in the cytoplasm of jujube and sour jujube were carried out based on thedetermination of aconitidase,and a comparison was made.It is believed that the activityof aconitidase is related to the quality of jujube and sour jujube.Ciaconitase belongs toferrothione.It has a [Fe.S.]in its body,which can transform citric acid into sodiumisocitric acid through the tricarboxylic acid cycle.ACO converts citric acid into isocitricacid,and the oxidative decarboxylation on isocitric acid makes the absorption peak ofNADH significantly enhanced at 240 nm.Through the analysis of the above results,themain conclusions of this paper are drawn.Our research group's previous studies have shown that there is a representativetype of "transition type"in the production of red dates in China,and this type of"transition type"plays a key role in the transformation of red dates to citric acid.On thisbasis,through comparative analysis,the commonalities and differences betweenhawthorn and hawthorn are elucidated,laying a theoretical foundation for the breedingand domestication of hawthorn.Key Words:Jujube;Shunaconitidase;Citric acid;Isocitric acid;Tricarboxylic acid cycle的湖北民族大学本科毕业论文目录摘要.IABSTRACT....III一、文献综述.…(一)枣和酸枣果实研究简介(二)乌头酸酶简介...。2(三)柠檬酸简介...(四)三羧酸循环简介…3二、乌头酸酶的提取与制备..·4(一)材料与方法....(二)实验方法..三、结果与分析..6(一)实验结果.....6(二)数据分析....结论10参考文献.....1113