碳源对灵芝深层发酵菌丝形态、多糖及三萜合成的影响研究

AbstractGanoderma lucidum is a famous medicinal fungus in China.The most importantpharmacodynamic components of G.lucidum extract are Ganoderma lucidumpolysaccharide and triterpenes,which are effective anti-tumor components.DPPHmethod was used to study the antioxidant ability of polysaccharides,triterpenes andfermentation broth during liquid fermentation of G.lucidum.As a result,the numberof mycelial spheres in the fermentation process of G.lucidum was the most,and 98/mL mycelial spheres.the biomass of the base was the heaviest ,2.54gL;theextracellular polysaccharide content in ganoderma lucidum fermentation process wasthe highest in fructose and lactose medium,0.47 g/L;the intracellular polysaccharidecontent in G.lucidum fermentation process was the highest in lactose medium 2.66gL;the triterpenoid content in ganoderma fermentation process was the highest inglucose medium,0.32%;the DPPH antioxidant content in the fermentation processwas the highest in lactose plus sucrose medium 17.23%.This paper can provide atheoretical reference for the economical and effective liquid fermentation of G.lucidum.Keywords:Ganoderma lucidum;liquid fermentation;polysaccharide;triterpene;mycelium biomass