纤维素降解菌的筛选-1

AbstractBiological composting is the main method for the utilization of solid wasteresources in most farms.It has the advantages of low investment,high efficiency,and high efficiency in the reuse of organic solid waste.However,there are alsosome shortcomings in the composting process,such as long fermentation cyclesand Odor,incomplete decomposition of cellulose and lignin,etc.Cow manurecontains a large amount of undigested cellulose and hemicellulose,which are thekey factors that affect the maturity of compost and determine the quality of thecompost product.Therefore,selecting and adding appropriate compostingfermentation inoculants and high-efficiency cellulose degrading inoculants in thecattle manure composting fermentation is an effective way to solve the problems oflong composting cycle of livestock and poultry manure and low degradation rate.In this study,enrichment culture and pure culture methods were used to isolatecellulose-degrading bacteria from cow manure and analyze their degradationcharacteristics,in order to obtain some high-efficiency cellulose-degrading bacteria.Using hydrolysis circle method and 3,5-dinitrosalicylic acid (DNS)method forstrain screening and enzymatic characteristics research,combined withmorphological methods for preliminary identification,molecular biologicalmethods to identify its species,a total of Three strains with good degradationeffects were named DH01,DH02,and DC02.Among them,DH01 is Bacillussubtilis with the highest cellulase activity;DH02 is Bacillus thuringiensis,