剩余污泥中蛋白质的提取及测定

剩余污泥中蛋白质的提取及测定摘要随着污水处理厂的不断建设，污水处理厂产生的剩余污泥，已经成为污水处理厂面临的主要问题之一。剩余污泥含有大量的有机物，其中蛋白质的含量丰富，蛋白质的提取及回收利用，能够实现固体废弃物的二次利用，同时有利于剩余污泥减量化、无害化和资源化。本研究选用热碱法提取剩余污泥中的蛋白质，应用考马斯亮蓝法测定水解液中蛋白质的含量，基于单因素实验，采用响应面法中的Box-Behnken中心组合设计实验原理，以温度、pH、水解时间作为响应因素，蛋白质提取含量作为响应值，对剩余污泥中蛋白质的提取条件进行优化。实验结果表明，最佳提取工艺条件为温度：75℃、pH12、水解时间4h时，蛋白质含量为3.016mgg。关键词：剩余污泥：蛋白质：污泥水解：响应面法ABSTRACTWith the continuous construction of sewage treatment plants,the treatment of excessactivated sludge produced during sewage treatment process has become one of the mainproblems.The excess activated sludge contains a large amount of organic matter,for exampleprotein,and the extraction and recycling of proteins can realize the reuse of solid waste,and atthe same time,it is conducive to the reduction,harmlessness and resourcefulness of excessactivated sludge.In this study,the protein in the excess activated sludge was extracted by hotalkaline method,and the protein content in the hydrolysate was determined by Caumas BrilliantBlue method.Based on the one-factor experiment,the extraction process of protein in the excessactivated sludge was optimized by using the experimental principle of Box-Behnken centralcombinatorial design,with the temperature,pH,and hydrolysis time as the corresponding factors,and the protein extraction content as the response value.The results showed that the optimalextraction process conditions:temperature 75C,pH12,and hydrolysis time 4h resulted in aprotein content of 3.016 mg/g.Key words:Excess activated sludge;Protein;Sludge hydrolysis;Response surface methodology目录第1章]引言…第2章材料与方法.22.1试剂与仪器….22.2买验万法.32.2.1标准曲线的绘制32.2.2标准曲线准确度测定32.2.2.1稳定性试验..32.2.2.2精密度试验。。2.2.2.3重现性试验…42.2.3蛋白质的提取及测定….42.2.4蛋白质提取的单因素实验设计42.2.4.1温度对蛋白质提取的影响2.2.4.2pH对蛋白质提取的影响.42.2.4.3水解时间对蛋白质提取的影响.52.2.5响应面法优化提取条件5第3章结果与分析.63.1标准曲线绘制63.2标准曲线准确度的测定.73.2.1稳定性试验.73.2.2精密度试验.83.2.3重现性试验3.3蛋白质提取单因素实验结果….83.3.1温度对蛋白质提取的影响…83.3.2pH对蛋白质提取的影响.93.3.3水解时间对蛋白质提取的影响.103.4响应面法确定三因素对蛋白质提取的交互效果.103.4.1响应面模型建立与分析103.4.2响应面最优化分析结果预测与验证.14Ⅲ第4章结论14参考文献.16致谢.18