厚叶岩白菜bHLH基因鉴定及分析研究

目录摘要。Abtract.1前言1.1厚叶岩白菜的生物学特性和价值1.2bHLH基因结构特点及功能研究1.3研究目的及意义2材料与方法、32.1实验材料2.2 BcbHLH基因的鉴定32.3 BebHLH的理化性质分析与亚细胞定位预测2.4 BcbHLH的系统进化树分析2.5 BebHLH的MEME分析与多序列比对2.6 BcbHLH的表达分析…3结果与分析3.1 BcbHLH基因注释筛选3.2 BcbHLH的理化性质分析与亚细胞定位预测.43.3 BebHLH的系统进化树分析3.4 BcbHLH蛋白Moif分析与多序列比对.83.5 BcbHLH基因表达分析..94讨论与结论.…4.1讨论..114.2结论…12参考文献.13致谢…15厚叶岩白菜bLH基因鉴定及分析研究摘要目的基于厚叶岩白菜根和叶转录组测序数据，本研究对厚叶岩白菜b亚H基因进行鉴定和表达分析。方法本研究基于厚叶岩白菜的测序数据，对厚叶岩白菜bH基因进行鉴定、生物学信息分析，包括理化性质、亚细胞定位、系统发育、保守基序等，并对其进行表达分析。结果本研究共鉴定到44个具有完整bHLH保守结构域的BcbHLH基因。BcbHLH中富含酸性氨基酸，41个BcbHLH为不稳定蛋白，均为亲水性蛋白。BcbHLH的进化树显示其可分为17个亚家族，BcbHLH蛋白与AtbHLH蛋白具有有较高的保守性。大部分BcbHLH的保守基序以Moif1和Moif2为主。BcbHLH基因表达在根和叶中存在差异，4个基因在根中表达较高，6个基因在叶中表达较高。结论本研究共鉴定到44个Bb亚H基因，为进一步深入探究厚叶岩白菜bHLH基因的功能研究奠定了理论基础。关键词：厚叶岩白菜：b亚H基因：进化树：表达分析Identification and Analytical Research of bHLH Gene inBergenia crassifolia (L.)FritschAbstractObjective Based on the transcriptome sequencing data of roots and leaves of Bergeniacrassifolia (L.)Fritsch,BcbHLH genes were identified and there expressions were analyzed.Methods This study was based on transcriptome data,identified the bHLH genes and analyzedthere biological information,including physical and chemical properties,subcellular localization,phylogeny,conserved moieties,etc.,and analyzed there expressions.Results 44 BcbHLH geneswith complete bHLH conserved domain were identified in this study.BcbHLH were rich in acidproteins proportion,41 BcbHLH were unstable proteins,all of them were hydrophilic proteins.Theevolutionary tree showed that the BcbHLH could be divided into 17 subfamilies.BcbHLH proteinswere highly conserved with Arabidopsis bHLH proteins.Most of the conserved motifs of BcbHLHwere Motif 1 and Motif 2.There were differences in BcbHLH genes expressions between roots andleaves.4 genes were highly expressed in roots and 6 genes were highly expressed in leaves.Conclusion 44 BcbHLH genes were identified in this study,which laid a theoretical foundation forfurther study on the function of bHLH genes in Bergenia crassifolia (L.)Fritsch.Keywords:Bergenia crassifolia (L.)Fritsch;bHLH gene;Evolutionary tree;Expressionanalysis