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根据GenBank中已经发表的牛TLR2、TLR4的cDNA序列，设计特异性引物及TaqMan探针，成功地扩增出基因表达水平可以用于定量实验的片段，建立了灵敏、准确性、稳定性均较好的检测荧光定量RT-PCR方法...
