蛹虫草双向发酵及抗氧化活性比较

蛹虫草双向发酵及抗氧化活性比较摘要本实验选取山药、茯苓、黄芪、姜黄、野菊花、北沙参、鱼腥草和金银花共八种中药作为发酵基质，分别接入蛹虫草菌丝体后进行双向发酵培养，获得发酵菌质。采用超声波法提取发酵菌质中的虫草素，采用水提醇沉法提取发酵菌质中的多糖，并对其多糖含量及抗氧化活性进行比较，结果发现：山药、北沙参、鱼腥草、黄芪、茯苓、姜黄与蛹虫草的双向发酵菌质多糖含量有所增加，且均高于中药以及蛹虫草菌丝体中多糖含量，双向发酵体系中以山药为基质的发酵菌质中多糖含量最高为12.61mgg:通过进一步的抗氧化活性测定表明，以山药为基质的发酵菌质多糖抗氧化活性最高，DPPH自由基清除能力高达86.11%,羟基自由基清除能力达到63.08%，ABTS自由基清除能力达到78.27%，超氧阴离子自由基的清除能力达到68.82%，均优于蛹虫草多糖。关键词：蛹虫草：抗氧化：双向发酵ABSTRACTIn this experiment,eight kinds of traditional Chinese medicines,namely,yam,poria,astragalus,turmeric,wild chrysanthemum,northern ginseng,fishy grass and honeysuckle,wereselected as fermentation substrates,and the fermentation mycelia of Cordyceps chrysanthemiwere inserted and cultured in both directions to obtain fermentation mycoplasm.Thepolysaccharide content and antioxidant activity of the fermentation mycelia were compared byusing ultrasonic method to extract cordycepin from the fermentation mycelia and aqueousalcoholic precipitation method,and the results showed that the polysaccharide content of thebi-directional fermentation mycelia of yam,northern ginseng,fishoil,astragalus,poria,turmericand chrysalis increased and were higher than those of the mycelia of Chinese medicine andchrysalis.The polysaccharide content of the fermentation mycelium with yam as the substrate inthe two-way fermentation system was 12.61 mg/g,and further antioxidant activity measurementshowed that the polysaccharide content of the fermentation mycelium with yam as the substratehad the highest antioxidant activity and was better than that of Chrysanthemum.In thisbidirectional fermentation system,the scavenging ability of its mycoplasmic polysaccharideDPPH free radicals reached 86.11%,hydroxyl radicals 63.08%,ABTS free radicals 78.27%,while its scavenging ability of superoxide anion free radicals reached 68.82%.Key words:Cordyceps militaris;Antioxidant;Bidirectional fermentationⅡ目录第1章引言….1第2章材料与方法.…22.1材料与设备...。2.1.1实验材料...22.1.2实验仪器...2.2实验方法2.2.1双向发酵菌质的制备...32.2.1.1菌种活化32.2.1.2发酵基质制备..42.2.1.3接种培养.....2.2.2虫草素含量测定........2.2.2.1虫草素标准曲线的绘制..…42.2.2.2虫草素的提取....…42.2.2.3虫草素含量的测定.....2.2.3多糖含量测定...·…52.2.3.1葡萄糖标准曲线的绘制....52.2.3.2水提醇沉法提取发酵菌质多糖...............52.2.3.3粗多糖含量测定..2.2.4抗氧化性测定.…S2.2.4.1DPPH自由基清除能力测定..............52.2.4.2羟基自由基清除能力测定...............52.2.43ABTS自由基(ABTS+)清除能力测定.·62.2.4.4超氧阴离子自由基清除能力测定.............6第3章结果与分析3.1蛹虫草对不同中药基质的生长适应性..3.2虫草素标准曲线的绘制......73.3双向发酵菌质中虫草素含量比较..73.4葡萄糖标准曲线的绘制..·….83.5双向发酵菌质中多糖含量比较...3.6双向发酵菌质多糖抗氧化性比较....Ⅲ